{"id":1815,"date":"2019-10-29T10:43:14","date_gmt":"2019-10-29T09:43:14","guid":{"rendered":"https:\/\/www.newslab.sk\/2019\/10\/29\/experimentalna-terapia-kmenovymi-bunkami-je-perspektivna-v-liecbe-chronickeho-diabetickeho-vredu-pripadova-studia\/"},"modified":"2019-11-04T14:03:21","modified_gmt":"2019-11-04T13:03:21","slug":"experimentalna-terapia-kmenovymi-bunkami-je-perspektivna-v-liecbe-chronickeho-diabetickeho-vredu-pripadova-studia","status":"publish","type":"post","link":"https:\/\/www.newslab.sk\/en\/experimentalna-terapia-kmenovymi-bunkami-je-perspektivna-v-liecbe-chronickeho-diabetickeho-vredu-pripadova-studia\/","title":{"rendered":"Experimental therapy with stem cells is promissing in the treatment of chronic diabetic ulcer \u2013 a case study"},"content":{"rendered":"

*<\/strong>All tables, charts, graphs and pictures that are featured in this article can be found in the .pdf attachment at the end of the paper.<\/strong><\/span><\/p>\n

 <\/p>\n

\u00davod<\/strong><\/h3>\n

Diabetes mellitus patr\u00ed k z\u00e1va\u017en\u00fdm metabolick\u00fdm chorob\u00e1m s vysok\u00fdm v\u00fdskytom v popul\u00e1cii. Prevalencia diabetu mellitu v Slovenskej republike sa za posledn\u00fdch 30 rokov takmer strojn\u00e1sobila. V Eur\u00f3pe patr\u00edme ku krajin\u00e1m s najvy\u0161\u0161\u00edm v\u00fdskytom tejto choroby(1). Aj ke\u010f s\u00fa k dispoz\u00edcii mo\u017enosti farmakologickej lie\u010dby, s oh\u013eadom na rast\u00facu incidenciu diabetu a predl\u017eovanie \u017eivota diabetikov sa hlavnou hrozbou st\u00e1vaj\u00fa chronick\u00e9 komplik\u00e1cie diabetu. Tie s\u00fa sprev\u00e1dzan\u00e9 vysok\u00fdmi n\u00e1kladmi na lie\u010dbu a v neposlednom rade s\u00fa zodpovedn\u00e9 za zv\u00fd\u0161en\u00fa mortalitu diabetick\u00fdch pacientov. Jedn\u00fdm z hlavn\u00fdch a najd\u00f4le\u017eitej\u0161\u00edch cie\u013eov mana\u017ementu diabetu je preto prevencia vzniku a progresie vaskul\u00e1rnych a neuropatick\u00fdch komplik\u00e1ci\u00ed.<\/p>\n

Chronick\u00e9 komplik\u00e1cie vznikaj\u00fa v s\u00favislosti s postihnut\u00edm cievneho rie\u010diska pri dlhodobo neuspokojivo kontrolovanej hyperglyk\u00e9mii. Na ich rozvoji sa podie\u013ea aj porucha metabolizmu lipidov, arteri\u00e1lna hypertenzia, inzul\u00ednov\u00e1 rezistencia a obezita, ktor\u00e9 s\u00fa spolu zahrnut\u00e9 do jednotky zvanej metabolick\u00fd syndr\u00f3m. Kardiovaskul\u00e1rne riziko je u pacientov s diabetom dvoj a\u017e \u0161tvorn\u00e1sobne vy\u0161\u0161ie v porovnan\u00ed s celkovou popul\u00e1ciou. Diabetick\u00e1 makroangiopatia vedie k akceler\u00e1cii ateroskler\u00f3zy ve\u013ek\u00fdch a stredne ve\u013ek\u00fdch tepien. Pri diabetickej mikroangiopatii vznikaj\u00fa komplexn\u00e9 funk\u010dn\u00e9 a \u0161truktur\u00e1lne zmeny ved\u00face k diabetickej nefropatii, retinopatii a neuropatii. \u0160t\u00fadie ukazuj\u00fa, \u017ee v \u010dase diagnostiky diabetu m\u00e1 u\u017e 10 % pacientov diabetick\u00fa nefropatiu, 6 % diabetick\u00fa retinopatiu, a\u017e 52 % diabetick\u00fa neuropatiu(2). Celkovo sa mikrovaskul\u00e1rne komplik\u00e1cie u asymptomatick\u00fdch pacientov s novozisten\u00fdm diabetom vyskytuj\u00fa takmer v tretine pr\u00edpadov(3).<\/p>\n

Medzi \u010dast\u00e9 komplik\u00e1cie pokro\u010dil\u00e9ho diabetu patr\u00ed aj diabetick\u00e1 noha. T\u00e1 je pod\u013ea WHO definovan\u00e1 ako infekcia, ulcer\u00e1cia a\/alebo de\u0161trukcia tkan\u00edv nohy v d\u00f4sledku neuropatie a\/ alebo angiopatie s isch\u00e9miou. Napriek pokrokom v diagnostike a lie\u010dbe diabetick\u00fdch komplik\u00e1ci\u00ed je lie\u010dba chronick\u00fdch vredov n\u00e1ro\u010dn\u00e1, nie v\u017edy \u00faspe\u0161n\u00e1 a viac ako jedna tretina pr\u00edpadov vedie k potrebe amput\u00e1cie postihnut\u00fdch kon\u010dat\u00edn(4). Diabetick\u00e1 noha m\u00e1 z\u00e1va\u017en\u00fd medic\u00ednsky dosah, ovplyv\u0148uje kvalitu \u017eivota pacientov a mo\u017enosti pracovn\u00e9ho a spolo\u010densk\u00e9ho uplatnenia. M\u00e1 z\u00e1sadn\u00fd vplyv na zv\u00fd\u0161enie finan\u010dn\u00fdch a person\u00e1lnych n\u00e1rokov na zdravotn\u00fd syst\u00e9m a ovplyv\u0148uje soci\u00e1lne prostredie pacienta.<\/p>\n

\u0160tandardn\u00e1 lie\u010dba diabetick\u00fdch vredov nohy pod\u013ea s\u00fa\u010dasn\u00fdch lie\u010debn\u00fdch protokolov zah\u0155\u0148a najm\u00e4 od\u013eah\u010dovanie nohy, chirurgick\u00e9 vy\u010distenie rany a obnovenie prekrvenia. Klinick\u00fd efekt takejto lie\u010dby je limitovan\u00fd a komplikovan\u00fd \u010dast\u00fdmi recid\u00edvami. H\u013eadanie nov\u00fdch met\u00f3d terapie chronick\u00fdch diabetick\u00fdch vredov je d\u00f4le\u017eit\u00fdm cie\u013eom s\u00fa\u010dasn\u00e9ho v\u00fdskumu. Ako jedna zo s\u013eubn\u00fdch met\u00f3d sa ukazuje lie\u010dba diabetickej nohy kme\u0148ov\u00fdmi bunkami(5). Kme\u0148ov\u00e9 bunky s\u00fa schopn\u00e9 ovplyvni\u0165 abnorm\u00e1lne zmenen\u00e9 mechanizmy hojenia a poru\u0161en\u00fa signaliz\u00e1ciu buniek v diabetick\u00fdch ran\u00e1ch a podporova\u0165 hojenie. Lie\u010dba je zalo\u017een\u00e1 na mezenchym\u00e1lnych kme\u0148ov\u00fdch bunk\u00e1ch a na predpoklade, \u017ee tieto bunky produkuj\u00fa modul\u00e1tory, ktor\u00e9 ovplyv\u0148uj\u00fa z\u00e1palov\u00fa reakciu aj rastov\u00e9 faktory potrebn\u00e9 na regener\u00e1ciu tkan\u00edv. Mechanizmus \u00fa\u010dinku t\u00fdchto buniek spo\u010d\u00edva v tom, \u017ee priamo tlmia z\u00e1palov\u00fd proces zn\u00ed\u017een\u00edm sekr\u00e9cie proz\u00e1palov\u00fdch a zv\u00fd\u0161en\u00edm produkcie protiz\u00e1palov\u00fdch cytok\u00ednov. Mezenchym\u00e1lne kme\u0148ov\u00e9 bunky vylu\u010duj\u00fa nieko\u013eko rastov\u00fdch faktorov, a t\u00fdm podporuj\u00fa prolifer\u00e1ciu fibroblastov v rane, angiogen\u00e9zu a ukladanie kolag\u00e9nu(6,7). V s\u00fa- \u010dasnosti sa uzn\u00e1va aj ich antimikrobi\u00e1lna aktivita(8).<\/p>\n

Cie\u013eom predlo\u017eenej pr\u00edpadovej \u0161t\u00fadie bolo overi\u0165 mo\u017enosti vyu\u017eitia experiment\u00e1lnej terapie kme\u0148ov\u00fdmi bunkami v lie\u010dbe diabetick\u00e9ho vredu v podmienkach Slovenskej republiky.<\/p>\n

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Pr\u00edpadov\u00e1 \u0161t\u00fadia<\/strong><\/p>\n

Prezentujeme pr\u00edpadov\u00fa \u0161t\u00fadiu 51-ro\u010dnej \u017eeny s diagn\u00f3zou diabetes mellitus 2. typu a s diabetickou ulcer\u00e1ciou na \u013eavej nohe pod MTTP V zhybom v trvan\u00ed 3 roky, lie\u010denou \u0161tandardn\u00fdmi lie\u010debn\u00fdmi postupmi po\u017e\u00edvan\u00fdmi v terapii chronick\u00fdch vredov (od\u013eah\u010denie kon\u010datiny, chirurgick\u00e9 vy\u010distenie rany, lok\u00e1lna a celkov\u00e1 ATB lie\u010dba, vlhk\u00e9 prev\u00e4zov\u00e9 techniky, kompenz\u00e1cia diabetu).<\/p>\n

Pred aplik\u00e1ciou kme\u0148ov\u00fdch buniek boli pacientke uroben\u00e9 \u0161tandardn\u00e9 laborat\u00f3rne odbery, bolo realizovan\u00e9 vy\u0161etrenie MRI na vyl\u00fa\u010denie osteomyelit\u00eddy skeletu, ster z rany na bakteriologick\u00e9 vy\u0161etrenie a exc\u00edzia z okraja vredu na histologick\u00e9 vy\u0161etrenie.<\/p>\n

Pou\u017eit\u00e9 kme\u0148ov\u00e9 bunky poch\u00e1dzali z pupo\u010dn\u00edkovej krvi, ktor\u00e1 sa odobrala prvorodi\u010dk\u00e1m rodiacim cis\u00e1rskym rezom. Vzorky buniek boli spracovan\u00e9 do 4 hod\u00edn po odbere. Tehotn\u00e9 darkyne boli testovan\u00e9 na prenosn\u00e9 choroby HBV, HCV, HIV a syfilis, CMV (s negat\u00edvnymi v\u00fdsledkami na testovan\u00e9 infekcie). Izolovan\u00e9 bunky boli kultivovan\u00e9 a otestovan\u00e9 na pr\u00edtomnos\u0165 bakt\u00e9ri\u00ed, h\u00fab a endotox\u00ednov.<\/p>\n

Mezenchym\u00e1lne kme\u0148ov\u00e9 bunky boli pacientke aplikovan\u00e9 ako dve lie\u010debn\u00e9 d\u00e1vky vo forme nieko\u013ek\u00fdch injekci\u00ed do rany a do bezprostrednej oblasti rany. Interval medzi dvoma podaniami bol t\u00fd\u017ede\u0148. V priebehu ka\u017edej lie\u010dby sa podali bunky v objeme 2,5 ml s po\u010dtom buniek 2 x 108 injek\u010dne.<\/p>\n

Pacientka bola sledovan\u00e1 v intervaloch 1, 2 a 3 mesiace po aplik\u00e1cii mezenchym\u00e1lnych kme\u0148ov\u00fdch buniek, s vidite\u013en\u00fdm zmen\u0161ovan\u00edm ko\u017en\u00e9ho defektu, s postupnou reepiteliz\u00e1ciou rany (obr\u00e1zok 1) <\/em><\/strong>aj s redukciou bakteri\u00e1lneho os\u00eddlenia rany. Do\u0161lo k vymiznutia mas\u00edvnej koloniz\u00e1cie rany Staphylococcus aureus<\/em>. Z v\u00fdsledkov realizovanej histol\u00f3gie bol evidentn\u00fd vplyv na akceler\u00e1ciu angiogen\u00e9zy, tvorbu granula\u010dn\u00e9ho tkaniva s jeho vyzrievan\u00edm.<\/p>\n

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Diskusia<\/h3>\n

Mezenchym\u00e1lne kme\u0148ov\u00e9 bunky predstavuj\u00fa skupinu multipotentn\u00fdch buniek s vysok\u00fdm potenci\u00e1lom v regenerat\u00edvnej medic\u00edne. Z\u00edskavaj\u00fa sa najm\u00e4 z pupo\u010dn\u00edkovej krvi a z hum\u00e1nnej placenty. Bunky maj\u00fa potenci\u00e1l diferenci\u00e1cie na v\u00e4\u010d\u0161inu mezoderm\u00e1lnych buniek(9,10), a t\u00fdm m\u00f4\u017eu prispie\u0165 <\/i>k efekt\u00edvnej\u0161ej tvorbe granula\u010dn\u00e9ho tkaniva, lep\u0161iemu hojeniu r\u00e1n aj k efekt\u00edvnej\u0161ej revaskulariz\u00e1cii tkaniva(11).<\/p>\n

Prezentovan\u00e1 \u0161t\u00fadia dokumentuje pozit\u00edvny \u00fa\u010dinok aplik\u00e1cie mezenchym\u00e1lnych kme\u0148ov\u00fdch buniek v lie\u010dbe diabetickej nohy, a to u\u017e v priebehu 1 mesiaca. Podobn\u00e9 v\u00fdsledky prezentovali aj in\u00ed autori(12,13). Pod\u00e1vanie mezenchym\u00e1lnych kme\u0148ov\u00fdch buniek malo pozit\u00edvny \u00fa\u010dinok na podporu revaskulariz\u00e1cie hojaceho sa diabetick\u00e9ho vredu a tvorbu granula\u010dn\u00e9ho tkaniva. Diabetes vedie k pred\u013a\u017eeniu a zv\u00fdrazneniu z\u00e1palovej f\u00e1zy a k poruch\u00e1m vaskulariz\u00e1cie(14). Pr\u00e1ve porucha tvorby granula\u010dn\u00e9ho tkaniva, a to najm\u00e4 jeho vyzrievania, sprev\u00e1dzan\u00e1 zn\u00ed\u017eenou produkciou kolag\u00e9nu, sa v experimentoch ukazuje ako d\u00f4le\u017eit\u00fd faktor pri zhor\u0161ovan\u00ed hojenia r\u00e1n pri diabete(15).<\/p>\n

Publikovan\u00e9 vedeck\u00e9 pr\u00e1ce ukazuj\u00fa, \u017ee pod\u00e1vanie mezenchym\u00e1lnych kme\u0148ov\u00fdch buniek je sprev\u00e1dzan\u00e9 aj siln\u00fdm antimikrobi\u00e1lnym \u00fa\u010dinkom, ktor\u00fd je \u010diasto\u010dne mediovan\u00fd vylu\u010dovan\u00edm chemok\u00ednov, lok\u00e1lnych medi\u00e1torov imunitnej reakcie a \u010fal\u0161\u00edch antimikrobi\u00e1lne p\u00f4sobiacich peptidov. Ukazuje sa, \u017ee mezenchym\u00e1lne kme\u0148ov\u00e9 bunky maj\u00fa pozit\u00edvny protiz\u00e1palov\u00fd \u00fa\u010dinok v experiment\u00e1lnych modeloch sepsy v in vitro, ako aj in vivo experimentoch(8). U\u017e tieto v\u00fdsledky predur\u010duj\u00fa ich mo\u017en\u00fd pozit\u00edvny \u00fa\u010dinok v lie\u010dbe chronick\u00fdch, \u0165a\u017eko sa hojacich r\u00e1n. Pr\u00e1ve s\u00fa\u010dasn\u00e1 produkcia rastov\u00fdch faktorov s vplyvom na angiogen\u00e9zu a v\u00fdznamn\u00fd antimikrobi\u00e1lny\u00a0 \u00fa\u010dinok\u00a0 predstavuj\u00fa\u00a0 ve\u013emi\u00a0 zauj\u00edmav\u00fa\u00a0 kombin\u00e1ciu\u00a0 s oh\u013eadom na vyu\u017eitie v lie\u010dbe \u0165a\u017eko sa hojacich diabetick\u00fdch vredov predkolenia(5).<\/p>\n

\u0160t\u00fadium mo\u017enost\u00ed vyu\u017eitia mezenchym\u00e1lnych kme\u0148ov\u00fdch buniek je v s\u00fa\u010dasnosti predmetom intenz\u00edvneho v\u00fdskumu. Pod\u013ea datab\u00e1zy klinick\u00fdch sk\u00fa\u0161ok spravovanej americk\u00fdm N\u00e1rodn\u00fdm in\u0161tit\u00fatom zdravia (clinicaltrials.gov) od\u0161tartovalo, prebieha alebo v ned\u00e1vnej minulosti prebehlo viacero klinick\u00fdch \u0161t\u00fadi\u00ed aplik\u00e1cie kme\u0148ov\u00fdch buniek u pacientov<\/p>\n

s diabetom mellitom, z toho 31 \u0161t\u00fadi\u00ed sa t\u00fdka priamo diabetickej nohy. Pod\u013ea metaanal\u00fdzy 6 klinick\u00fdch \u0161t\u00fadi\u00ed Guo et al.(16) kon\u0161tatuj\u00fa, \u017ee aplik\u00e1cia kme\u0148ov\u00fdch buniek zlep\u0161ila proces hojenia, a to aj pri l\u00e9zi\u00e1ch v\u00e4\u010d\u0161\u00edch ako 5 cm2, aj vo vekovej skupine nad 70 rokov.\u00a0 Anal\u00fdza v\u0161ak pouk\u00e1zala na viacer\u00e9 limit\u00e1cie t\u00fdchto \u0161t\u00fadi\u00ed. Doposia\u013e nebol vytvoren\u00fd vhodn\u00fd jednotn\u00fd protokol odpor\u00fa\u010danej lie\u010dby. Napriek viacer\u00fdm u\u017e ukon\u010den\u00fdm klinick\u00fdm \u0161t\u00fadi\u00e1m ch\u00fdbaj\u00fa ucelen\u00e9 z\u00e1very a odpor\u00fa\u010dania, ktor\u00e9 by bolo mo\u017en\u00e9 priamo aplikova\u0165 do praxe. Spolo\u010dn\u00fdm menovate\u013eom realizovan\u00fdch \u0161t\u00fadi\u00ed je taktie\u017e aplik\u00e1cia samostatn\u00fdch kme\u0148ov\u00fdch buniek.<\/p>\n

V\u00fdsledky realizovanej pr\u00edpadovej \u0161t\u00fadie potvrdzuj\u00fa perspekt\u00edvne pozit\u00edvne vplyvy pod\u00e1vania mezenchym\u00e1lnych kme\u0148ov\u00fdch buniek v lie\u010dbe diabetick\u00fdch ulcer\u00e1ci\u00ed. Zauj\u00edmav\u00e1 by mohla by\u0165 s\u00fa\u010dasn\u00e1 aplik\u00e1cia \u010fal\u0161\u00edch podporn\u00fdch lie\u010debn\u00fdch met\u00f3d vr\u00e1tane larv\u00e1lnej, pr\u00edpadne f\u00e1govej terapie. V s\u00fa\u010dasnosti pokra\u010dujeme v \u010fal\u0161om v\u00fdskume, ktor\u00fd by zah\u0155\u0148al aj tieto met\u00f3dy.<\/p>\n

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\u0160t\u00fadia bola realizovan\u00e1 s podporou projektu APVV-17-0526<\/em><\/p>\n

\u201eVyu\u017eitie mezenchym\u00e1lnych kme\u0148ov\u00fdch buniek v kombin\u00e1cii s \u010fal\u0161\u00edmi podporn\u00fdmi biologick\u00fdmi postupmi v lie\u010dbe chronick\u00e9ho diabetick\u00e9ho vredu\u201c a je s\u00fa\u010das\u0165ou pr\u00e1ce v\u00fdskumn\u00e9ho centra vybudovan\u00e9ho v\u010faka podpore v r\u00e1mci OP V\u00fdskum a v\u00fdvoj pre projekt: \u201eDobudovanie multidisciplin\u00e1rneho centra pre biomedic\u00ednsky v\u00fdskum \u2013 BIOMEDIRES\u201c, ITMS 26210120041, spolufinancovan\u00fd zo zdrojov Eur\u00f3pskeho fondu region\u00e1lneho rozvoja.<\/em><\/p>\n

 <\/p>\n

LITERAT\u00daRA<\/strong><\/p>\n

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  13. Cao Y, Gang X, Sun C, et Mesenchymal Stem Cells Improve Healing of Diabetic Foot Ulcer. J Diabetes Res 2017; 2017: 9328347.<\/li>\n
  14. Tengrup I, Hallmans G, Agren Granulation tissue formation and metabolism of zinc and copper in alloxan-diabetic rats. Scand J Plast Reconstr Surg Hand Surg 1988; 22(1): 41-5.<\/li>\n
  15. Yue DK, Swanson B, McLennan S, et al. Abnormalities of granulation tissue and collagen formation in experimental diabetes, uraemia and malnutrition. Diabet Med 1986; 3(3): 221-5.<\/li>\n
  16. Guo J, Dardik A, Fang K, et Meta-analysis on the treatment of diabetic foot ulcers with autologous stem cells. Stem Cell Res Ther 2017; 8(1): 228.<\/li>\n<\/ol>\n","protected":false},"excerpt":{"rendered":"

    *All tables, charts, graphs and pictures that are featured in this article can be found in the .pdf attachment at the end of the paper.   \u00davod Diabetes mellitus patr\u00ed k z\u00e1va\u017en\u00fdm metabolick\u00fdm chorob\u00e1m s vysok\u00fdm v\u00fdskytom v popul\u00e1cii. Prevalencia diabetu mellitu v Slovenskej republike sa za posledn\u00fdch 30 rokov takmer strojn\u00e1sobila. V Eur\u00f3pe patr\u00edme<\/p>\n","protected":false},"author":7,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_mi_skip_tracking":false,"footnotes":""},"categories":[1299],"tags":[734,1303,1304],"class_list":["post-1815","post","type-post","status-publish","format-standard","hentry","category-nove-metody-liecby-en","tag-diabetes-mellitus","tag-diabetic-foot","tag-mesenchymal-stem-cells","typ_clanku-original-work"],"acf":{"abstrakt":"

    Diabetes mellitus is a frequent disease in the population associated with serious complications. The vascular and neuropathic complications are significantly responsible for the morbidity and mortality of diabetic patients and accompanied by high treatment expenses. The diabetic foot is one of the most severe complications that often lead to limb loss. The currently available routine treatment is often not sufficiently effective. The search\u00a0 for new methods of chronic diabetic ulcer therapy is an important task of contemporary research. The use of mesenchymal stem cells has proven to be a promising method of treating diabetic foot. Despite several clinical studies, there are still no clear recommendations for practice. We present a case study, in which the aim was to verify the possibilities of using experimental stem cell therapy in the treatment of diabetic ulcer in the Slovak Republic. The success of the applied treatment has opened up the possibilities of a broader clinical study that\u00a0 is currently underway.<\/p>\n

    Keywords: <\/strong>diabetes mellitus, diabetic foot, mesenchymal stem cells<\/p>\n","casopis":[{"ID":1883,"post_author":"7","post_date":"2019-10-28 13:35:57","post_date_gmt":"2019-10-28 12:35:57","post_content":"

      \r\n \t
    • Detection and validation of subchromosomal aberrations detected as additional findings in routine noninvasive prenatal testing for common trisomies<\/li>\r\n \t
    • Finding of a marker chromosome in a child with Cat-eye syndrome (case study)<\/li>\r\n \t
    • Experimental therapy with stem cells is promissing in the treatment of chronic diabetic ulcer \u2013 a case study<\/li>\r\n \t
    • Colonic mucosal Schwann cell hamartoma with tactile corpuscle-like bodies: a case report<\/li>\r\n \t
    • Accidental capture of the toxic strain Corynebacterium diphteriae<\/li>\r\n<\/ul>","post_title":"newsLab","post_excerpt":"","post_status":"publish","comment_status":"closed","ping_status":"closed","post_password":"","post_name":"newslab-004","to_ping":"","pinged":"","post_modified":"2019-11-04 13:53:35","post_modified_gmt":"2019-11-04 12:53:35","post_content_filtered":"","post_parent":0,"guid":"https:\/\/www.newslab.sk\/casopis\/newslab-4\/","menu_order":0,"post_type":"casopis","post_mime_type":"","comment_count":"0","filter":"raw"}],"strana":"77-79","upload_clanok":{"ID":1813,"id":1813,"title":"NEWSLAB_2-2019_Lang","filename":"NEWSLAB_2-2019_Lang.pdf","filesize":163904,"url":"https:\/\/www.newslab.sk\/wp-content\/uploads\/2019\/10\/NEWSLAB_2-2019_Lang.pdf","link":"https:\/\/www.newslab.sk\/en\/experimentalna-terapia-kmenovymi-bunkami-je-perspektivna-v-liecbe-chronickeho-diabetickeho-vredu-pripadova-studia\/newslab_2-2019_lang-2\/","alt":"","author":"7","description":"","caption":"","name":"newslab_2-2019_lang-2","status":"inherit","uploaded_to":1815,"date":"2019-10-29 09:33:16","modified":"2019-10-29 09:33:16","menu_order":0,"mime_type":"application\/pdf","type":"application","subtype":"pdf","icon":"https:\/\/www.newslab.sk\/wp-includes\/images\/media\/document.png"}},"_links":{"self":[{"href":"https:\/\/www.newslab.sk\/en\/wp-json\/wp\/v2\/posts\/1815","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.newslab.sk\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.newslab.sk\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.newslab.sk\/en\/wp-json\/wp\/v2\/users\/7"}],"replies":[{"embeddable":true,"href":"https:\/\/www.newslab.sk\/en\/wp-json\/wp\/v2\/comments?post=1815"}],"version-history":[{"count":0,"href":"https:\/\/www.newslab.sk\/en\/wp-json\/wp\/v2\/posts\/1815\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.newslab.sk\/en\/wp-json\/wp\/v2\/media?parent=1815"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.newslab.sk\/en\/wp-json\/wp\/v2\/categories?post=1815"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.newslab.sk\/en\/wp-json\/wp\/v2\/tags?post=1815"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}